attomol« Borrelia burgdorferi Realtime LT

REF 1136

20 reactions

Intended purpose

The kit attomol® Borrelia burgdorferi-Realtime LT enables the qualitative detection of Borrelia burgdorferi-DNA (sensu lato). Moreover, it is a manual, LoopTag probe based real-time PCR kit designed for LightCycler® 1.x or 2.0. The sample material has to be liquor cerebrospinalis, synovial fluids, skin biopsies, or ticks.

Field of application

The bite of a tick infected with Borrelia burgdorferi (Bb) may cause clinical symptoms of Lyme disease [Braune H.J., 1991, Fortschr. Neurol. Psychiatr., 59: 456-467]. First symptoms appear after an incubation time of 2-32 days in the form of a typical rose disease. The most frequent sites of this lesion are the upper leg, the groin, and the shoulder. In a considerable number of patients, these symptoms combined with stage 1 of the disease are missed completely [Hechemy K.E., 1986, Bull. Soc. Pathol. Exot. Filiales 79: 9-21]. In the 2nd stage of the disease the pathogen may attack other organs. Symptoms range from headache, neck stiffness, fever, shaking chills, transient muscular pain, exhaustion, and general indisposition to arthralgia. Early symptoms of the Lyme disease are typically intermittent. After some weeks or months neurological symptoms such as menigoradiculitis, encephalomyelitis, and neuritis appear and may be accompanied by radicular pain as a first symptom. Within weeks, sensibility disturbances and cerebral nerve deficiencies (e.g. facial paresis) and paresis of the extremities may also occur. Early neurological findings recede within months but chronic symptoms may appear in the future [Isselbacher et al, 1995, Blackwell-Wissenschaftsverlag, 13. Auflage  890f.]. The late stage of the Lyme disease (stage 3) is characterised by a third leading symptom, acrodermatitis chronica atrophicans [Braune H.J., 1991, Fortschr. Neurol. Psychiatr., 59: 456-467]. The disease attacks especially women at the age of 50-70. In addition, the majority of insufficiently treated patients develop arthritis, mostly in large joints (e.g. knees). The detection of Borrelia in patient’s Liquor cerebrospinalis samples or synovial fluids is not possible at all time during the course of an infection. Therefore, negative findings do not exclude a Borrelia infection and other criteria such as clinical symptoms or serum antibodies should be taken into consideration.

Kit components
Technology
  • Oligomix B. burgdorferi RT LT
  • Interner Standard B. burgdorferi RT LT
  • Instructions for use

You can find more informations about the Looptag-Technology here.

Short informations
Sample
DNA from
  • ticks
  • skin biopsies
  • synovial fluids
  • liquor cerebrospinalis
Devices
LightCycler« 1.x
LightCycler« 2.0
Duration
ca. 1,5 h
Determinations
20 reactions
Annex

The Instructions for use and the Material safety data sheet can be accessed via the login area. This area is conserved for Attomol customers. Please log in with your customer number and the corresponding password. For further informations pleaseácontact us directly.