attomol^® Fibrinogen alpha Quicktype

The  assay  attomol^®  Fibrinogen  alpha  Quicktype  is  used  for  the  supportive  diagnosis  of  thrombophilia.  This test can be used to determine the polymorphism Thr312Ala in the human fibrinogen alpha gene (FGA gene, rs6050). It is a manual, qualitative assay using an allel-specific PCR with integrated probe hybridisation. The evaluation takes place by agarose gel electrophoresis, which can be carried out manually or automatically. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

Fibrinogen is an important acute-phase coagulation factor which is divided to fibrin monomers and polymers by thrombin. Fibrin monomers and polymers were connected and stabilised by factor XIII which enables the formation of a coagulation thrombus. Abnormalities in this system could cause bleeding or thrombosis. Fibrinogen  consists  of  three  pairs  of  polypeptide  chains  (Aα/Bβ/γ)2.  In  the  fibrinogen  α-gene  (FGA-gene)  a base  exchange  from  adenine  to  guanine  (6534A>G)  could  appear  which  is  defined  as  single  nucleotide polymorphisms  (SNP)  rs6050  [Siegerink et al., Journal of Thrombosis and Haemostasis 2008, 7:385-390].  Hereby,  in  the  amino acid  position  312,  alanine  instead  of  threonine (Thr312Ala) is integrated into the α-fibrinogen protein. The Thr312Ala polymorphism lies in a region which is important for the factor XIII dependent coagulation process. Through this, both the thickness of the fibrin fiber and the stability of the network can be affected [Standeven et al., Circulation 2003, 107:2326-2330]. Several studies show coherence between the Th312Alapolymorphism and pulmonary embolism as well as an association to a higher mortality rate after stroke [Suntharalingam et al., European Respiratory Journal 2008, 31:736-741; Carter et al., Circulation 1999, 99:2423-2426]. Because of these facts the mutation becomes interesting for the clinical risk estimation of the named diseases. In the Caucasian population the allele frequency for this polymorphism is approximately 40 % [Carter et al., Blood 2000, 96:1177-1179].

• PCR-H₂O
• PCR-buffer I
• primer FGA Thr312Ala
• instructions for use

You can find more informations about Quicktype-Technology here.