attomol® Fibrinogen beta Quicktype
REF 1263
20 reactions
Intended purpose
The assay attomol® Fibrinogen beta Quicktype is used for the supportive diagnosis of thrombophilia. This test can be used to determine the transition -455G>A in the human fibrinogen beta gene (FGB gene, rs1800790). It is a manual, qualitative assay using an allel-specific PCR with integrated probe hybridisation. The evaluation takes place by agarose gel electrophoresis, which can be carried out manually or automatically. The sample material has to be genomic DNA prepared from EDTA or citrate blood.
Field of application
Fibrinogen is a coagulation factor consisting of three polypeptide chains (alpha, beta, gamma), which is converted to fibrin by thrombin. Fibrin is an essential component for the formation of a coagulation thrombus [Hoppe, B., 2014, Thromb Haemost., 112, 649-658]. Abnormalities in this system can lead to an increased risk of thrombosis [de Willige et al., 2005, Blood, 106, 4179-83]. In the review by Iacoviello et al. it is reported on the basis of various publications that transversion -455G>A in
the promoter region of the fibrinogen beta gene is associated with an increased fibrinogen concentration [Iacoviello et al., 2001, Hypertension, 38, 1199-1203].
Also in the editorial of Papageorgiou et al. the mutation -455G>A is described as the most important genetic polymorphism associated with increased fibrinogen concentration [Papageorgiou et al., 2010, Hellenic J Cardiol, 51: 1-9]. Patients with a fibrinogen concentration greater than 5 g/L were observed to have a 4-fold increase in the risk of thrombo-embolism [Koster T. et al., 1994, Thromb Haemost., 71, 719-722]. The mutated allele for the polymorphism -455G>A in the fibrinogen beta gene occurs with a prevalence of about 22 % in the European population [U.S National Library of Medicine, National Center for Biotechnology Information: SNP Datenbank, Link: „https://www.ncbi.nlm.nih.gov/snp/rs18000790/#frequency_tab“, Zugriffsdatum: 28.03.19].
Kit content
Technology
- PCR-H2O
- PCR-buffer VI
- Primer Fibrinogen beta QT
- Instructions for use
Mehr Informationen zur Quicktype-Technologie finden Sie hier.
Short informations
DNA from blood
Thermal cycler
Gel or capillariy electrophoresis system
3-4 h
20 reactions
Annex
The Instructions for use and the Material safety data sheet can be accessed via the login area. This area is conserved for Attomol customers. Please log in with your customer number and the corresponding password. For further informations please contact us directly.