attomol^® GST P1 Quicktype

The assay attomol^® GST P1 Quicktype is used for the supportive diagnosis to assess the detoxification capacity of  the  organism'.  This  test  can  be  used  to  determine the  GST  P1  polymorphism  (Ile105Val,  rs1695)  of  the glutathione S-transferase genes. It is a manual, qualitative assay using an allel-specific PCR with integrated probe  hybridisation.  The  evaluation  takes  place  by  agarose  gel  electrophoresis,  which  can  be  carried  out manually or automatically. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

Glutathione  S-transferases  (GST)  belong  to  enzymes of  phase  II  metabolism.  They  conjugate  electrophilicforeign substances or their metabolites with L-glutathione. In most cases the reaction has a detoxifying effect, but can also lead to metabolic activation [Schulz et al., 2002, Umweltmed Forsch Prax, 7(4):232-246]. For the majority of GST sequence variations (polymorphisms) are known, including the variations GST M1, GST T1 and GST P1. Polymorphism GST P1 is based on a single-point mutation that affects a changed enzyme activity. The base exchange (A to G) causes an amino acid exchange from Isoleucine to Valine at position 105 of the protein backbone [Dufour et al., 2005, Haematologica/the Hematology Journal, 90(8):1027-1031]. Dependent on the substrate, the enzyme activity can be reduced or increased, which can have a variety of clinical consequences. The significance of the GST P1 polymorphism in the context of  an  increased  cancer  risk,  environmental  medical  issues  and  the  metabolism  of  certain  drugs  is controversially discussed in the literature in analogy to the GST M1 and GST T1 zero genotypes. In different extrahepatic tissues GST P1 is expressed and metabolises for example substances like acrolein and chlordinitrobenzol.  The  homozygous  mutation  (Val/Val)  exists  in  different  ethnic  groups  with  frequencies ranging between 5 and 15 % dependent on the population analysed.

• PCR-H₂O
• PCR-premix GST P1
• instructions for use

You can find more informations about Quicktype-Technology here.