attomol® Lactose Intolerance Duplex Realtime LT

REF 1186

20 reactions

REF 1234

100 reactions

Intended purpose

The assay attomol® Lactose intolerance Duplex Realtime LT is used for the supportive diagnosis of lactose intolerance. This test can be used for the simultaneous determination of the transitions -13910C>T (rs4988235) and -22018G>A (rs182549) in the regulatory range of the human lactase gene (MCM6 gene). It is a manual, qualitative real-time PCR assay based on LoopTag probes, which is evaluated by a melting curve analysis. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

Field of application

The uptake of milk or other lactose-containing foods causes intolerances in some adults. These are often assigned to the syndrome of primary (congenital) lactose intolerance (LIT) manifested in adulthood. The most frequent symptoms are lactose maldigestion, meteorism (tympanites), distension and diarrhoea [Obermayer-Pietsch, 2004, Journal für Mineralstoffwechsel, 11(3):20-23]. Lactose tolerance may be caused by activating mutations (-13910C>T, -22018G>A) in the MCM6 gene (minichromosome maintenance gene) on chromosome 2, near the lactase gene (lactase phlorizin hydrolase gene, LPH gene) [Olds und Sibley, 2003, Hum Mol Genet, 12(18):2333-2340]. Activating mutation denotes a mutation where the carrier receives a biological advantage compared to the non-mutated individual. In this example, it is the tolerance towards lactose. Besides the mutation -13910C>T also the polymorphism -22018G>A is considered important for the diagnosis of lactose intolerance Bulhoes et al., 2007, Braz J Med Biol Res, 40(11):1441-1446]. Homozygous carriers of the mutations (-13910TT, -22018AA) have a livelong lactose tolerance. In heterozygous carriers it is supposed that the inactivity of the one allele may be partially compensated [Obermayer-Pietsch, 2004, Journal für Mineralstoffwechsel, 11(3):20-23; Sibley, 2004, Am J Pharmacogenomics, 4(4):239-245]. The presence of the wild type (-13910CC, -22018GG) causes a reduced lactase activity, which decreases even further with increasing age. As a consequence of the reduced lactase activity lactose is not cleaved effectively into the monosaccharides glucose and galactose. Because not-cleaved lactose can not be reabsorbed by the human organism the symptoms mentioned above can typically be found [Srinivasan und Minocha, 1998, Postgrad Med, 104(3):109-111, 115-116, 122-123]. The loss of lactase activity usually starts after the ablactation phase and ends in adult at the status of lactose intolerance. The prevalence of the lactose intolerance varies worldwide strongly between different ethnical groups. In the European population, with a prevalence of less than 30 %, the lactose intolerance is not widely distributed [Sahi, 1994, Scand J Gastroenterol, 202(29):7-20]. In Austria for example, 20-25 % of the people suffer from the primary adult lactose intolerance [Obermayer-Pietsch, 2004, Journal für Mineralstoffwechsel, 11(3):20-23].

Kit content
  • Oligomix Lactose Intolerance Duplex LT
  • instructions for use

You can find more informations about the Looptag-Technology here.

Short informations
DNA from blood
LightCycler® 1.x
LightCycler® 2.0
LightCycler® 480
ca. 1,5 h
20 reactions
100 reactions

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