attomol« MTHFR 677C>T Realtime LT

REF 1171

40 reactions

REF 1222

160 reactions

Intended purpose

The assay attomol® MTHFR 677C>T Realtime LT is used for the supportive diagnosis of thrombophilia. This test can be used to determine the transition 677C>T (rs1801133) in the human MTHFR gene. It is a manual, qualitative real-time PCR assay based on a LoopTag probe, which is evaluated by a melting curve analysis. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

Field of application

The methylenetetrahydrofolate reductase (MTHFR) catalyses the reaction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, which functions as the methyl donor for the conversion of homocysteine to methionine. The mutation 677C>T causes production of a thermolabile enzyme with reduced enzymatic activity [Huisman, M. V., Rosendaal, F.; (1999) Thrombophilia. Current Opinion in Hematology 6:291-297]. As a result reduced conversion of homocysteine to methionine leads to an accumulation of homocysteine in the plasma (hyperhomocysteinaemia). Enhanced plasma levels of homocysteine are known to be a risk factor for thrombosis and artherosclerosis, especially if combined with mutations in the blood clotting factors V and II (e.g. prothrombin mutation, Leiden mutation) [Bertina, R.M.; (1998) Current Opinion in Hematology 5:339-342].
The homozygous form of the MTHFR gene mutation 677C>T is carried by 11 % of the European population. The inheritance of more than one genetic risk factor can lead to an early and pronounced manifestation of thrombosis.

Kit content
Technology
  • Oligomix MTHR 677C>T LT
  • instructions for use

You can find more informations about the Looptag-Technology here.

Short informations
Sample
DNA from blood
Devices
LightCycler« 1.x
LightCycler« 2.0
LightCycler« 480
Duration
ca. 1,5 h
Determinations
40 reactions
160 reactions
Annex

The Instructions for use and the Material safety data sheet can be accessed via the login area. This area is conserved for Attomol customers. Please log in with your customer number and the corresponding password. For further informations pleaseácontact us directly.