attomol« Mycobacterium tuberculosis Realtime LT 2

REF 1190

20 reaction

Intended purpose

The assay attomol® Mycobacterium tuberculosis Realtime LT 2 is used to detect pathogens of Mycobacterium tuberculosis complex (MTC = M. tuberculosis, M. bovis, M. africanum, M. microti). The test requires DNA isolated from sputum. It is a manual, qualitative test for the supportive diagnostics of tuberculosis.

Field of application

Tuberculosis is one of the most common diseases, infecting more than 8 million people annually and causing about 2 million deaths [Loddenkemper et al., 2002, Pneumologie, 56:550-557]. The disease is mainly caused by two pathogens - by Mycobacterium tuberculosis and more rarely by Mycobacterium bovis. In Africa, however, M. africanum is often the trigger for tuberculosis [Haas et al., 1997, J Clin Microbiol., 35: 663-666]. In very rare cases, M. microti was also described as the cause [Niemann et al., 2000, Emerg. Infect. Dis., 6 (5): 538-542]. Tuberculosis commonly affects the lung but basically can affect all organs. The transmission of the infection happens over the pulmonary tracts in human. Entry of the pathogen into the host initiates an unspecific inflammatory reaction with slight or no symptoms, which is rarely recognised. Pathogenetically, two forms of tuberculosis can be distinguished. The first form is primary tuberculosis, which occurs after an intial infection. This form is frequent during childhood and is mostly located within the lung (lung tuberculosis). The disease starts with the formation of a primary infection site and the distribution of the pathogens to the regional lymph nodes. The primary tuberculosis is accompanied by slight symptoms as slight fever, body weight reduction, higher tendency to sweat, tiredness, loss of appetite. It heals completely in 90 % of all cases as a result of effective immune reaction. In 10 % of all cases a secondary tuberculosis develops immediately or after several years. This second form of the tuberculosis may attacks the whole bronchial system or other organs by spreading the bacteria from primary or earlier infection sites throughout the organism [Kayser et al., 1998, Medizinische Mikrobiologie, 9. überarbeitete und neugestaltete Auflage, Georg-Thieme-Verlag Stuttgart - New York, S. 256ff].
The course of tuberculosis depends on the amount and on the virulence of the pathogen as well as on the self-protective power of the host (immunity, resistance). The clinical pattern of tuberculosis may very considerably, which makes a proper diagnosis indispensable. Beside the classic in vitro culture, the PCR has been demonstrated to be a very sensitive and fast diagnostic tool. PCR-based direct pathogen detection for the fast diagnosis in liquor samples has special significance as well in patients suspected to be newly infected.

Kit components
Technology
  • Oligomix Mycobacterium tuberculosis LT 2
  • Internal standard Mycobacterium tuberculosis LT 2
  • Instructions for use

You can find more informations about the Looptag-Technology here.

Short informations
Sample
DNA from sputum
Devices
LightCycler« 2.0
LightCycler« 480
Duration
ca. 1,5 h
Determinations
20 reactions
Annex

The Instructions for use and the Material safety data sheet can be accessed via the login area. This area is conserved for Attomol customers. Please log in with your customer number and the corresponding password. For further informations pleaseácontact us directly.