attomol Autoantibody Beadassay

  • Bead based multiparameter detection of antibodies
  • Microtest plate format
  • Semiautomatic or automatic
  • Semiquantitative method

The development of the technology is finished and first applications are about to be launched.

Introduction

The Antibody Beadassay is a multiplex test system for a simultaneous detection of different antibodies in human serum. Our technology applies to differential diagnostics for autoimmune diagnostics as well as infectious serology. The processing of the indirect immunoassay can be realized by standard ELISA automats.

Test principle
Attomol Beadassay Technology
Attomol Beadassay Technology

The Antibody Beadassay is a fluorescence-based detection method which allows the parallel detection of antibodies in human serum samples. Antigenes are bound to size- and fluorescence-coded beads which are immobilized at the bottom of a conventional microplates' wells. The antibodies of the sample serum bind to the corresponding beads. After incubation with a fluorescently marked secondary antibody, the fluorescent beads are measured with the Caleidoscan 100. With both the size and fluorescence of the beads taken into account, the exact antigene-mapping is performed according to positive or negative antibody binding.

Required devices
Results

The evaluation of the fluorescence measuring is carried out by the attomol® Caleidopro 1.x software. It calculates semiquantitative fluorescence ratios from referenced fluorescence intensities. Based on this, the investigated samples are diagnosed accorrding to specific thresholds as negative, borderline or positive.

Attomol Beadassay Technologie