attomol Autoantibody Lineassay

  • strip based multiparameter detection of antibodies
  • established and robust method
  • economically efficient
Introduction

Our autoantibody Lineassay technology applies to the detection of antibodies for autoimmune diagnostics. With these multiplex tests certain human antibodies can be determined which plays a major role for peripheral neuropathies (ganglioside-ab) or for disruptions of the blood clotting system. Our test kits enable the optional determination of either IgG- or IgM-antibodies in full scale of the declared determinations.

Test principle
Attomol Lineassay technology
Attomol Lineassay technology

During the first step of incubation antigenes, which are immobilized on the test strip, are binded by specific antibodies of the sample. Non-binded sample material is removed by a first washing step. These, now immobilized, human primary antibodies are binded specifically by antihuman-Ig-secundary antibodies, which are conjugated with the enzym peroxidase, during the second step of incubation. Unbinded conjugate is removed by a second washing phase. The colorless substrate solution is enzymatical converted to a dark purple precipitate by peroxidase. The precipitate now binds, due to its hydrophope characteristic, on the hydrophope test strip neare to its genisis. This reaction is stoped by a third washing step.

Required devices
Schüttler Gerät

seesaw shaker
(40 U/min)

Scanner, Gerät

scanner (for archiving)

Results

The autoantibody Lineassay technology provides qualitative results. The staining intensity of the test strips is compared to the thresholds on an interpretation template. The evaluation of results includes the categories negative, borderline, and positive.