attomol Autoantibody Lineassay

  • Strip based multiparameter detection of antibodies
  • Established and robust method
  • Economically efficient
Introduction

Our Autoantibody Lineassay technology is designed to detect antibodies in the course of autoimmune diagnostics. With these multiplex tests, human antibodies can be determined in peripheral neuropathies (ganglioside antibodies) or disruptions of the blood clotting system. Our test kits facilitate optional determination of either IgG- or IgM-antibodies.

Test principle
Attomol Lineassay technology
Attomol Lineassay technology

During the first step of incubation, antigenes which are immobilized on the test strip bind specific antibodies of the sample. Unbound sample material is removed by a first washing step. In the next step of incubation, the now immobilized human primary antibodies are specifically bound by anti-human Ig secondary antibodies which are conjugated with the enzyme peroxidase. Unbound conjugate is removed in a second washing phase. The colourless substrate solution is enzymatically converted to a dark purple precipitate by peroxidase. Because of its hydrophobic characteristics, this precipitate now binds close to its formation on the hydrophobic test strip. This reaction is stoped by a third washing step.

Required devices
Schüttler Gerät

Seesaw shaker
(40 U/min)

Scanner, Gerät

Scanner (for archiving)

Results

The Autoantibody Lineassay technology provides qualitative results. The staining intensity of the test strips is compared to thresholds on an interpretation template. The evaluation of results includes the categories negative, borderline, and positive.