attomol® Realtime TM
- Real time PCR
- Reliable TaqManTM based technology
- Adapted and standard real time cyclers
![TaqMan Technologie](uploads/c/technologiebild-mtm-250x250-0.jpg)
Introduction
For diagnostics of thrombophilia, metabolism and immunogenetics, we offer real time PCR assays based on TaqManTM technology for the detection of disease-related polymorphisms in the human genome. This is a fast and safe procedure which facilitates reliable genotyping of patients' samples on various devices.
![attomol Realtime TM Kit](uploads/c/aufnahme-1-250x250-0.png)
Test kits
products
Test principle
![Attomol Realtime TM Technology](uploads/realtime-tm-technologiebild-1-en.gif)
![Attomol Realtime TM Technology](uploads/realtime-tm-technologiebild-2-en.gif)
![Attomol Realtime TM Technology](uploads/realtime-tm-technologiebild-3-en.gif)
![Attomol Realtime TM Technology](uploads/realtime-tm-technologiebild-4-en.gif)
The items of this product line are based on the TaqManTM principle: the specific TaqManTM probe is hybridised on the wild type or the mutated allele degrades by 5′-nuclease activity of the polymerase within each amplification cycle. The released fluorescent dyes can be detected in the corresponding channel of a real time thermal cycler.
Equipment
LightCycler® 480 (Roche)
Mx3005P, AriaMx (Agilent Technologies)
Rotor-Gene® Q (Qiagen)
CFX96TM (Biorad)
peqSTAR 96Q (VWR)
MIC (Bio Molecular Systems)
Results
The test can can be comfortably evaluated by means of a scatter plot (fig. C) or by allele specific comparing amplification curves (fig. A and B).
![Amplifikationskurzve TaqMan Technologie](uploads/c/tm-amplifikationskurve-a.png)
![Amplifikationskurve TaqMan Technologie](uploads/c/tm-amplifikationskurve-b.png)
![Ergebnis TaqMan Streudiagramm](uploads/c/tm-streudiagramm-c.png)