attomol® Factor II 19911A>G Quicktype

REF 1159

20 reactions

Intended purpose

The assay attomol® Factor II 19911A>G Quicktype is used for the supportive diagnosis of thrombophilia. This test can be used to determine the transition 19911A>G (rs3136516) in the human factor II gene (prothrombin mutation). It is a manual, qualitative assay using an allel-specific PCR with integrated probe hybridisation. The evaluation takes place by agarose gel electrophoresis, which can be carried out manually or automatically. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

Field of application

In the second-last step of the blood coagulation cascade, prothrombin (factor II) is converted into the serine protease  thrombin.  This  converts  fibrinogen  molecules by  splitting  the  fibrinopeptides  A  and  B  into  fibrin monomeres, which spontaneously aggregate to fibrin polymers. In  addition  to  the  polymorphism  20210G>A  in  prothrombin,  19911A>G  was  identified  as  a  second polymorphism in the same gene, which is also associated with an increase in prothrombin concentration in blood  plasma  and  thus  with  an  increased  risk  of  venous  thromboembolism  [Chinthammitr et al., J Thromb Haemost 2006, 4:2587-92].  The  mutated  allele  of polymorphism 19911A>G occurs in the European population with a prevalence of approximately 47 % [U.S  National  Library  of  Medicine, SNP-Database, 2019-09-19]. Furthermore,  it  was  found  that  the  19911A  allele  is  strictly  coupled  to  the  20210A  allele  [Zivelin et al., Blood 1998, 92:1119-24].  Especially in combination  with  other  polymorphisms  and  risk  factors a  determination  of  the  polymorphism'  19911A>G should be considered [Martinelli et al., J Thromb Haemost 2006, 4:2582-6].

Kit content
  • PCR-H2O
  • PCR-buffer
  • Primer Factor II 19911A>G
  • instructions for use

You can find more informations about Quicktype-Technology here.

Short informations
DNA from blood
Thermal cycler
Gel or capillariy electrophoresis system
3-4 h
20 reactions

The Instructions for use and the Material safety data sheet can be accessed via the login area. This area is conserved for Attomol customers. Please log in with your customer number and the corresponding password. For further informations please contact us directly.