attomol^® Factor II 20210G>A Realtime TM 2

The assay attomol^® Factor II 20210G>A Realtime TM 2 is used for the supportive diagnosis of thrombophilia. This test can be used to determine the transition 20210G>A in the human factor II gene (prothrombin mutation, rs1799963). It is a manual, qualitative, on hydrolysis probes based real-time PCR assay designed for various real-time PCR cyclers. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

In the second-last step of the blood coagulation cascade, prothrombin (factor II) is converted into the serine protease thrombin. This converts fibrinogen molecules by splitting the firbrinopeptides A und B into fibrin monomeres, which spontaneously aggregate to fibrin polymers. The replacement of guanine (G) at position 20210 of the prothrombin gene by adenine (A) (factor II [prothrombin] mutation) causes an increase in the prothrombin concentration in the blood plasma and thus promotes the development of thromboembolism [Poort et al., 1996, Blood 88: 3698-3703]. The incidence of this mutation is 1-3 % in the European population. In carriers of the heterozygous mutation in prothrombin gene, the risk of thrombosis in combination with other factors (intake of oral oestrogen-containing contraceptives, surgical operations, pregnancy, increased age, smoking) increases 3 to 5-fold compared with healthy people [Markis et al., 1997, Thromb. Haemost. 78:1426-1429].

• PCR-H₂O
• Premix Factor II 20210G>A TM 2
• Hot Start Taq DNA Polymerase (2 U/µl)
• instructions for use

You can find more informations about the TaqMan^TM-Technology here.