attomol® Factor II 20210G>A Realtime TM 2

REF 1247

48 reactions

REF 1248

96 reactions

Intended purpose

The assay attomol® Factor II 20210G>A Realtime TM 2 is used for the supportive diagnosis of thrombophilia. This test can be used to determine the transition 20210G>A in the human factor II gene (prothrombin mutation, rs1799963). It is a manual, qualitative, on hydrolysis probes based real-time PCR assay designed for various real-time PCR cyclers. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

Field of application

In the second-last step of the blood coagulation cascade, prothrombin (factor II) is converted into the serine protease thrombin. This converts fibrinogen molecules by splitting the firbrinopeptides A und B into fibrin monomeres, which spontaneously aggregate to fibrin polymers. The replacement of guanine (G) at position 20210 of the prothrombin gene by adenine (A) (factor II [prothrombin] mutation) causes an increase in the prothrombin concentration in the blood plasma and thus promotes the development of thromboembolism [Poort et al., 1996, Blood 88: 3698-3703]. The incidence of this mutation is 1-3 % in the European population. In carriers of the heterozygous mutation in prothrombin gene, the risk of thrombosis in combination with other factors (intake of oral oestrogen-containing contraceptives, surgical operations, pregnancy, increased age, smoking) increases 3 to 5-fold compared with healthy people [Markis et al., 1997, Thromb. Haemost. 78:1426-1429].

Kit content
  • PCR-H2O
  • Premix Factor II 20210G>A TM 2
  • Hot Start Taq DNA Polymerase (2 U/µl)
  • instructions for use

You can find more informations about the TaqManTM-Technology here.

Short informations
DNA from blood
LightCycler® 480
Rotor-Gene® Q
peqSTAR 96Q
ca. 1,5 h
48 reactions
96 reactions

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