attomol^® Factor V Leiden Realtime TM 2

The assay attomol^® Factor V Leiden Realtime TM 2 is used for the supportive diagnosis of thrombophilia. This test can be used to determine the transition 1691G>A in the human factor V gene (Leiden mutation, rs6025). It is a manual, qualitative, on hydrolysis probes based real-time PCR assay designed for various real-time PCR cyclers. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

The factor V Leiden mutation, which has a prevalence of approximately 5 % in the European population, is one of the most important hereditary risk factors for thrombosis. In the blood coagulation cascade factor V forms a complex together with the activated factor X, phospholipids, and calcium ions (prothrombin activator). This complex converts prothrombin (factor II) into thrombin, which causes a fibrin polymerisation. The inactivation of factor V occurs through the activated protein C (APC). The substitution of guanine (G) for adenine (A) at position 1691 of the factor V gene results in an amino exchange in the binding site for APC. This leads to the fact that factor V can only be insufficiently cleaved and thus inactivated [Bertina et al., 1994, Nature 369: 64-67]. The resulting accumulation of factor V in the blood leads to an increased coagulation tendency, which results in a higher risk of thrombosis in the affected patients. Homozygous mutations in the factor V gene lead to a significantly higher risk than heterozygous defects [Zöller et al., 1999, Haematologica 84: 59-70].

• PCR-H₂O
• Premix Factor V Leiden TM 2
• Hot Start Taq DNA Polymerase (2 U/µl)
• instructions for use

You can find more informations about the TaqMan^TM-Technology here.