attomol^® HLA-B*27 Realtime TM 2

The assay attomol^® HLA-B*27 Realtime TM 2 is used to determine the HLA-B*27-locus on the chromosome 6 in the human genome. It is used for supportive diagnosis of ankylosing spondylitis. Moreover, it is a manual, qualitative, on hydrolysis probes based real-time PCR assay designed for various real-time PCR cyclers. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

(Do not use for tissue typing!)

The human leukocyte antigens (HLA) are glycoproteins of the major histocompatibility complex (MHC), which are expressed on nearly all nucleated cells. These antigens are responsible for the immunological individuality of humans (differentation between endogenic, i.e. “internally generated” and exogenic, i.e. foreign). The HLA-B antigens belong together with HLA-A und HLA-C to the MHC I molecules and their genes are located on the short arm of chromosome 6 [Thomas, 2000, Labor und Diagnose, 5. erweiterte Auflage, TH-Books Verlagsgesellschaft mbH, S. 878-888]. There exist different forms of HLA-B antigens, the so-called determinants (e.g. HLA-B*7, -B*8, -B*15). One of these determinants - HLA-B*27 - was found in 1973 to be closely related to certain rheumatic diseases, especially to Spondylitis ancylosans (SPA, Morbus Bechterew) [Brewerton et al., 1973, Lancet 1:904-907]. Carriers of HLA-B*27 bear an 87-fold increase in the risk to suffer from SPA as carriers of other determinants. The disease manifests very often between the 15th and 30th year of life and in 90 % of all cases is found in men. HLA-B*27 occurs in about 7 % of the Western Europe population. Patients with SPA carry HLA-B*27 in 80-90 % of all cases [Isselbacher et al., 1995, Harrisons Innere Medizin 1, 13. Auflage, Blackwell-Wissenschaftsverlag, S. 453-460]. However, the pathogenesis is not completely understood [Taurog, J Rheumatol 2010, 37(12):2606-16]. Until now, HLA-B*27 was detected serologically. Recently, this method is increasingly replaced by molecular biological techniques, because molecular tests work without living cells and are cheaper and easier to perform.

• PCR-H₂O
• Premix HLA-B*27 TM 2
• Hot Start Taq DNA Polymerase (2 U/µl)
• instructions for use

You can find more informations about the TaqMan^TM-Technology here.