attomol« MTHFR 677C>T Realtime TM 2

REF 1253

48 reactions

REF 1254

96 reactions

Intended purpose

The assay attomol« MTHFR 677C>T Realtime TM 2 is used for the supportive diagnosis of thrombophilia. This test can be used to determine the transition 677C>T (rs1801133) in the human methylenetetrahydrofolate reductase (MTHFR) gene. It is a manual, qualitative, on hydrolysis probes based real-time PCR assay designed for various real-time PCR cyclers. The sample material has to be genomic DNA prepared from EDTA or citrate blood.

Field of application

The methylenetetrahydrofolate reductase (MTHFR) catalyses the reaction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, which functions as the methyl donor for the conversion of homocysteine to methionine. The mutation 677C>T causes production of a thermolabile enzyme with reduced enzymatic activity [Huisman, M. V., Rosendaal, F.; (1999) Thrombophilia. Current Opinion in Hematology 6:291-297]. As a result reduced conversion of homocysteine to methionine leads to an accumulation of homocysteine in the plasma (hyperhomocysteinaemia). Enhanced plasma levels of homocysteine are known to be a risk factor for thrombosis and artherosclerosis, especially if combined with mutations in the blood clotting factors V and II (e.g. prothrombin mutation, Leiden mutation) [Bertina, R.M.; (1998) Current Opinion in Hematology 5:339-342]. The homozygous form of the MTHFR gene mutation 677C>T is carried by 11 % of the European population. The inheritance of more than one genetic risk factor can lead to an early and pronounced manifestation of thrombosis.

Kit content
Technology
  • PCR-H2O
  • Premix MTHFR 677C>T TM 2
  • Hot Start Taq DNA-Polymerase (2 U/Ál)
  • instructions for use

You can find more informations about the TaqManTM-Technology here.

Short informations
Sample
DNA from blood
Devices
LightCycler« 480
AriaMx
Mx3005P
Rotor-Gene« Q
CFX96TM
peqSTAR 96Q
Duration
ca. 1,5 h
Determinations
48 reactions
96 reactions
Annex

The Instructions for use and the Material safety data sheet can be accessed via the login area. This area is conserved for Attomol customers. Please log in with your customer number and the corresponding password. For further informations pleaseácontact us directly.